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Yes No Customer Inquiry Form Member access Global --> Elitech Group SAS × Global USA France Australia & New-Zealand Benelux Italy Latin America (Brazil) UK Germany Menu Home 118金宝搏抽水 118金宝搏抽水 188abc金博宝 188188金宝搏 Careers CSR Policy Products 188bet无法提款 ELITe InGenius Instrument 精英InGenius菜单 ELITe BeGenius Instrument Open RT-PCR assays 168博金宝 Clinical Chemistry Chemistry Systems Chemistry Reagents Osmometry Chloridometers Microbiology Instrumentation Obstetric and Pregnancy Bacteriology Mycology Parasitology Quality Controls Serology Virology Hematology Slide Stainers Cytocentrifuge Erythrocyte Sedimentation Rate Cell Image Analyzers Cytology Slide stainers CF Sweat Testing Sweat Collection Sweat Analysis News Press Releases 188.博金宝 Blog Support 188金宝搏app安卓下载 Contact Contact Subsidiaries Operations ELITechGroup offices ELIchrom glabrata --> Products Microbiology Rapid ID tests Mycology Rapid test for the identification ofCandida glabrata. ELIchrom glabrata is a fast unitary test for the rapid identification of Candida glabrata colonies. Cost-effective, routine laboratory test Easy and ready-to-use High sensitivity and specificity Fast results for clinicians (20 minutes) Simple colorimetric reaction, without expert interpretation Request a quote ELIchrom glabrata Catalogue number(s) 44506Product Description: ELIchrom glabrataNumber of Tests: 40 tests Request a quote Downloads Yeast Rapid Test Brochure (EN) Features Specifications Related Products Intended use Candidas are usually commensal yeasts of the digestive and urogenital mucosas. They become pathogenic only when favorable conditions arise in the host. Studies show thatC. glabrata是第二大commonly encountered yeast in humans and its pathogenic nature is no longer disputed:C. glabratais responsible for 10 to 25% of all the candidemias. Moreover,C. glabratais generally less sensitive to azole derivatives, with certain isolates from patients treated with fluconazole or ketoconazole appearing to be resistant to virtually all the azole derivatives. The isolation and rapid identification ofC. glabratais thus essential to determine the correct therapeutic treatment. Traditionally, biochemical tests are used in the identification ofC. glabrata. The disadvantage of the carbon auxanogram and the zymogram is that there is a long 24 to 48 hours wait before the tests can be read. C. glabratacan be identified more quickly with systems based upon the detection of preformed enzymes that use chromogenic substrates or alternatively, that are based upon the assimilation of trehalose. ELIchromglabratais a rapidC. glabrataidentification test based upon the detection of trehalase. Principle C. glabratais able, under certain conditions, to rapidly hydrolyze trehalose into glucose. The detection of glucose production (by a revelator, which contains oxidase glucose, peroxidase and a chromogenic substrate) can therefore be used to positively identify this species. However, in addition to the trehalose test, two controls must also be carried out: A maltose control is necessary because certainC. tropicalisisolates can also degrade trehalose, but unlikeC. glabratathey also assimilate maltose. A control is carried out in the absence of monosaccharide (reagent base medium control). This is necessary because of the presence of glucose in isolation media. Simple protocol Add 100 µL of sterile distilled water to a haemolysis tube. Homogenize the colonies in the tube in order that the suspension density is equivalent to point 5 in the Mc Farland range (3-4 colonies if the culture has been carried out on Sabouraud medium and 3-8 colonies, according to the colonies size, if the culture has been carried out on a chromogenic medium). With a micropipette add 25 µL of the prepared yeast suspension to each blue spot in the T, M and B wells. Leave the test to incubate for 10 minutes at room temperature. With a micropipette add 25 µL of the reagent to each of the T, M and B wells. Incubate the test for 5 to 10 minutes at room temperature. Read the test results. Easy-to-read and easy-to-interpret results +: Appearance of a more or less dark orange coloration-: Absence of any coloration Results CONCLUSION WELL T (Trehalose) WELL M (Maltose) WELL B(Base Medium) + - - c . glabrata。 - - - NonC. glabratayeast. Continue the identification. + + - - + - + + + Non interpretable. Repeat the tests by carefully sampling the yeast without moving the agar. Reagents and Material REAG:Vial of lyophilized reagent (to be reconstituted with 1.3 mL of sterile distilled water) TEST CARD:Test cards containing 12 wells , 4 of which are for identification: T wells contain base medium and trehalose M wells contain base medium and maltose B wells contain base medium Quantity: 10 Stability and Storage Reagents stored at 2-8°C, in their original state, are stable up to the expiry date indicated on the box. They must not be frozen.The results of the stability study indicate that the product has a stability of 12 months at 2-8°C from its manufacture. It was also demonstrated that, after reconstitution, the REAG is stable 4 weeks at ambient temperature and 2 months between 2°-8°C. The shelf life of the reconstituted REAG may be extended to 6 months by freezing it (-20°C) in the form of 75 µL aliquots immediately after reconstitution. 性能 评价results for ELIchromglabratademonstrate, depending upon the isolation medium used, a sensitivity from 93.8 to 97.9% (97.9% for the Candida ID, CandiSelect, and Sabouraud media and 93.8% for the CHROMagar candida medium) and a specificity from 97.6 to 98.8% (98.8% for the Sabouraud and CHROMagar candida media, 98% for the Candida ID medium and 97.6% for the CandiSelect medium). Material required but not provided Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured Hemolysis tubes Sterile distilled water Pasteur pipette or wire loop Contaminated waste container CANDICHROM®II Pack Selective isolation of fungi and direct identification ofCandida albicans. CANDIFAST® Test combining identification and antifungal resistance testing of the main pathogenic yeasts. FUNGIFAST® Test combining identification and antifungal resistance testing of the main pathogenic yeasts. CANDIFAST®ES TWIN Antifungal resistance testing of yeasts responsible for superficial mycosis. FUNGIFAST®AFG Antifungal susceptibility testing of the main pathogenic yeasts. ELIchrom FUNGI Complete test for the identification of the main pathogenic yeasts. ELITex Bicolor albi-dubli Latex slide agglutination test for the rapid identification ofCandida albicans/Candida dubliniensisgroup. ELITex Bicolor dubliniensis Slide agglutination test for the rapid identification ofCandida dubliniensis. ELITex krusei Slide agglutination test for the rapid identification ofCandida krusei. ELI.H.A Candida Indirect hemagglutination test for serodiagnosis ofcandidiasis. ELI.H.A Aspergillus Indirect hemagglutination test for serodiagnosis ofAspergillosis How can we help you? 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